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. 2024 Dec 11;8(3):e202403122. doi: 10.26508/lsa.202403122

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© 2024 Kobia et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S7. — (A) A high-content S7 widefield image of MCF10A cells treated and immunolabeled as indicated. SGK3 silencing causes marked NOTCH1 accumulation in the cytoplasm. (B, C) RT-qPCR and Western blot analyses indicate effective SGK3 depletion. **** indicates P < 0.0001. (D) Western blot analyses indicate that SGK3 does not destabilize N1ICD or N1FL. (E) Western blot analyses of lysates treated with λ-phosphatase (λ-PPA) showed that similar to controls, most of the N1ICD that accumulates upon SGK3 silencing is phosphorylated.