TABLE 2.

Surface phenotypes of PBM cells from BLV⁻, AL, and PL cattle

Marker	Cell population (% ± SD)a
	BLV−		AL		PL
	Ex vivob	Culturedc	Ex vivo	Cultured	Ex vivo	Cultured
CD2	38 ± 8	72 ± 13	57 ± 11	72 ± 7	18 ± 1	48 ± 10
CD4	17 ± 4	19 ± 1	37 ± 8	38 ± 5	13 ± 4	24 ± 7
CD8	13 ± 7	47 ± 5	16 ± 4	11 ± 2de	7 ± 2	27 ± 8f
Surface IgM	30 ± 3	NDg	36 ± 5	15 ± 4	56 ± 8	28 ± 11
γδ-TcR	14 ± 2	21 ± 14	15 ± 3	41 ± 3de	9 ± 4	8 ± 2h

^aFor ex vivo cells, n = 10 for all groups. For cultured cells: BLV⁻, n = 3 (of 8); AL, n = 10 (of 10); PL, n = 10 (of 10). 

^bEx vivo cells are nonexpanded PBM cells freshly isolated from blood. 

^cNonadherent cells were cultured with endogenously expressed BLV antigen for 2 to 3 days and then rhIL-2 (50 U/ml) for an additional 7 to 9 days. 

^dStatistically different from BLV⁻ (P < 0.001). 

^eStatistically different from PL (P < 0.001). 

^fStatistically different from BLV⁻ (P < 0.005). 

^gND, not determined. 

^hNot statistically different from BLV⁻ (P > 0.025).