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. 2024 Dec 11;19(12):e0289769. doi: 10.1371/journal.pone.0289769

Table 2. Summary of macroscopic and microscopic features of bone alteration and their implications.

Bone alteration markers Thermal status Observations Marker for Implication
Macroscopic indicators
Fracture aspects Unburnt Three broken in wet condition Perimortem damage (environment, human) Strong application of force before skeletonisation complete
Colour Burnt Black (a few), white to grey-blue; variation within and between bones/individuals Thermal manifestation Different temperatures, (mostly) between 600° and 800°
Texture Burnt Chalky to firm; see above Thermal manifestation Different temperatures, (mostly) between 600° and 800°
Deformation Burnt Some warping Thermal manifestation Different temperatures, (mostly) between 600° and 800°
Fracture patterns Burnt Parabolic, splitting Thermal manifestation Dry or wet bone?
Fragment size Both 90% are >30% complete Thermal manifestation, taphonomic disturbance Size atypical for funeral cremation
Gnawing marks Both Absent Post-mortem damage (environment) Human remains were not encountered by scavenging animals
Root imprints Both Present on several bones Post-mortem damage (environment) Similar late post-mortem damage on all bones
Microscopic indicators
Histological preservation (OHI scale) Both 5 for burnt, 2–3 for unburnt Thermal manifestation; post-mortem damage (environment) Combustion soon after death; post-mortem diagenesis of original bone
Degree of collagen preservation Both Present in 10 of 10 thin-sections Thermal manifestation; bone tissue diagenetic processes Different temperatures, (mostly) between 600° and 800°
Trapped carbon Burnt Present in 6 of 7 thin-sections Thermal manifestation Different temperatures, (mostly) between 600° and 800°
Cracks, burst osteons Burnt Present in 6 of 7 thin-sections Thermal manifestation Different temperatures, (mostly) between 600° and 800°; wet-bone combustion
Microbial attack Unburnt Present in 3 of 3 thin-sections Post-mortem damage (environment) Post-mortem diagenesis of original bone

For diagnostic marker descriptions and implications, see Ellingham et al. [86]; Wahl [87]. For more examples, detailed descriptions, and illustrations, see Fig 6 and S1 Table.