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. 2024 Dec 11;13:RP102912. doi: 10.7554/eLife.102912

Figure 2. Novel class IIb microcins are effective inhibitors of Enterobacteriaceae and Gram-negative ESKAPE pathogens.

Figure 2.

(A) Heatmap summarizing the inhibitory potential of known and novel class IIb microcins against a library of Enterobacteriaceae, Pseudomonadales, and Gram-positive bacteria, including multidrug-resistant isolates (red) as determined by static inhibition assays with live-producing bacteria. *=activity determined through microcin purification and minimum inhibitory concentration assays (Mortzfeld et al., 2022; Palmer et al., 2020). (B) Relative minimum inhibitory concentrations for S. enterica (Se) G492 against different bacterial species. Note that Se G492 is 256 times more potent against A. baumannii (BAA 1790) compared to K. pneumoniae (BAA 1705). (C) Static inhibition assays comparing K. pneumoniae (Kp) E492, Kp G492, Ec H47, E. coli (Ec) I47, Ec M, and Se G492 activity from single colony production against multidrug-resistant A. baumannii (BAA 1790) and Pseudomonas aeruginosa (PA14). Note that iron-limited conditions (DP) are required for antimicrobial activity, confirming action of class IIb microcins. L-ara=L-arabinose, DP = 2,2-dipyridyl, scale bars: 1 cm.