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. 2000 Sep;74(18):8316–8323. doi: 10.1128/jvi.74.18.8316-8323.2000

TABLE 3.

Amino acid replacements found in VP1 and 3D of the mutant spectrum of FMDV populations subjected to chemical mutagenesis

Protein Amino acid replacementa Structural elementb Presence (+) or absence (−) of replacements in the following populationc:
FU AZC Other
VP1 K-41 → Ed βB + +
T-50 → A βC +
I-61 → V αA +
Y-72 → C βD +
P-94 → L l +
H-108 → Rd βF +
G-142 → W l +
T-148 → K l + +
T-149 → K l + + +
T-150 → K l + +
H-151 → R l + +
E-167 → D l +
E-167 → Kd l +
3D A-116 → T l +
V-181 → I l +
I-196 → T l +
F-230 → S l +
R-289 → C l +
I-334 → M β2 +
D-349 → E l +
a

The single-letter amino acid code is used; amino acids are numbered independently for each protein. 

b

Structural elements (α, α helix; β, β strand; l, loop) of VP1 are assigned according to the three-dimensional structure of FMDV C-S8c1 (30, 39); structural elements of 3D are based on the three-dimensional structure of poliovirus 3D (25), assuming that the equivalent positions in FMDV 3D in an alignment of the two sequences (37) have the same structure. 

c

FU and AZC, FMDV populations derived from C-S8c1 after one or multiple passages in the presence of FU and AZC, respectively; they correspond to the populations described in Tables 1 and 2. Other, possible presence of the replacements in populations of FMDV type C natural isolates (35, 36, 39) or laboratory variants of FMDV C-S8c1 (3, 19, 20, 27, 40, 59). 

d

These three replacements have been found in the mutant spectrum of some populations and are dominant in some consensus sequences: K-41 → E and H-108 → R in three out of five clones of C-S8c1FUp25 and E-167 → K in two out of five clones of C-S8c1FUp25. Minority amino acids which coincide with the amino acid in the corresponding position of C-S8c1 are not included.