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Kinetics of luciferase gene expression from dicistronic replicons PV-Luc-EMCV and EMCV-Luc-PV. Equivalent amounts of in vitro-transcribed RNA from pPV-Luc-EMCV (A) or pEMCV-Luc-PV (B) were transfected into HeLa cells previously infected with VV-P1 for 3 h. The cells were then incubated at the designated temperatures for the specified times. Cell lysates were prepared and analyzed for luciferase expression. Total RLU was plotted versus the same time posttransfection for each temperature.
