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. 1981 Dec 1;199(3):807–811. doi: 10.1042/bj1990807

Metalloproteinases from rabbit bone culture medium degrade types IV and V collagens, laminin and fibronectin.

G Murphy, T E Cawston, W A Galloway, M J Barnes, R A Bunning, E Mercer, J J Reynolds, R E Burgeson
PMCID: PMC1163439  PMID: 7041891

Abstract

Gel-filtration chromatography of culture medium from rabbit bone explants separates three latent metalloproteinases with activities against collagen, proteoglycan and gelatin respectively. The fractions degrading proteoglycan also degrade laminin, fibronectin and the polymeric products of pepsin-solubilized type IV collagen and can also solubilize insoluble type IV collagen. The fractions degrading gelatin are capable of degrading solubilized type V and 1 alpha,2 alpha,3 alpha (cartilage) collagens, as well as the lower-molecular-weight products of pepsin-solubilized type IV collagen. All activities can be inhibited by 1,10-phenanthroline and occur in either partially or totally latent forms that can be activated by 4-aminophenylmercuric acetate.

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Selected References

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  1. Bentz H., Bächinger H. P., Glanville R., Kühn K. Physical evidence for the assembly of A and B chains of human placental collagen in a single triple helix. Eur J Biochem. 1978 Dec;92(2):563–567. doi: 10.1111/j.1432-1033.1978.tb12778.x. [DOI] [PubMed] [Google Scholar]
  2. Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burgeson R. E., El Adli F. A., Kaitila I. I., Hollister D. W. Fetal membrane collagens: identification of two new collagen alpha chains. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2579–2583. doi: 10.1073/pnas.73.8.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burgeson R. E., Hollister D. W. Collagen heterogeneity in human cartilage: identification of several new collagen chains. Biochem Biophys Res Commun. 1979 Apr 27;87(4):1124–1131. doi: 10.1016/s0006-291x(79)80024-8. [DOI] [PubMed] [Google Scholar]
  5. Burleigh M. C., Barrett A. J., Lazarus G. S. Cathepsin B1. A lysosomal enzyme that degrades native collagen. Biochem J. 1974 Feb;137(2):387–398. doi: 10.1042/bj1370387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cambray G. J., Murphy G., Page-Thomas D. P., Reynolds J. J. The production in culture of metalloproteinases and an inhibitor by joint tissues from normal rabbits, and from rabbits with a model arthritis. I. Synovium. Rheumatol Int. 1981;1(1):11–16. doi: 10.1007/BF00541217. [DOI] [PubMed] [Google Scholar]
  7. Cawston T. E., Tyler J. A. Purification of pig synovial collagenase to high specific activity. Biochem J. 1979 Dec 1;183(3):647–656. doi: 10.1042/bj1830647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  9. Liotta L. A., Lanzer W. L., Garbisa S. Identification of a type V collagenolytic enzyme. Biochem Biophys Res Commun. 1981 Jan 15;98(1):184–190. doi: 10.1016/0006-291x(81)91886-6. [DOI] [PubMed] [Google Scholar]
  10. Liotta L. A., Tryggvason K., Garbisa S., Robey P. G., Abe S. Partial purification and characterization of a neutral protease which cleaves type IV collagen. Biochemistry. 1981 Jan 6;20(1):100–104. doi: 10.1021/bi00504a017. [DOI] [PubMed] [Google Scholar]
  11. Mainardi C. L., Dixit S. N., Kang A. H. Degradation of type IV (basement membrane) collagen by a proteinase isolated from human polymorphonuclear leukocyte granules. J Biol Chem. 1980 Jun 10;255(11):5435–5441. [PubMed] [Google Scholar]
  12. Mainardi C. L., Seyer J. M., Kang A. H. Type-specific collagenolysis: a type V collagen-degrading enzyme from macrophages. Biochem Biophys Res Commun. 1980 Dec 16;97(3):1108–1115. doi: 10.1016/0006-291x(80)91490-4. [DOI] [PubMed] [Google Scholar]
  13. Murphy G., Cambray G. J., Virani N., Page-Thomas D. P., Reynolds J. J. The production in culture of metalloproteinases and an inhibitor by joint tissues from normal rabbits, and from rabbits with a model arthritis. II. Articular cartilage. Rheumatol Int. 1981;1(1):17–20. doi: 10.1007/BF00541218. [DOI] [PubMed] [Google Scholar]
  14. Nagase H., Woessner J. F., Jr An improved assay for proteases and polysaccharidases employing a cartilage proteoglycan substrate entrapped in polyacrylamide particles. Anal Biochem. 1980 Sep 15;107(2):385–392. doi: 10.1016/0003-2697(80)90400-5. [DOI] [PubMed] [Google Scholar]
  15. Rhodes R. K., Miller E. J. Physicochemical characterization and molecular organization of the collagen A and B chains. Biochemistry. 1978 Aug 22;17(17):3442–3448. doi: 10.1021/bi00610a003. [DOI] [PubMed] [Google Scholar]
  16. Sage H., Bornstein P. Characterization of a novel collagen chain in human placenta and its relation to AB collagen. Biochemistry. 1979 Aug 21;18(17):3815–3822. doi: 10.1021/bi00584a027. [DOI] [PubMed] [Google Scholar]
  17. Sage H., Woodbury R. G., Bornstein P. Structural studies on human type IV collagen. J Biol Chem. 1979 Oct 10;254(19):9893–9900. [PubMed] [Google Scholar]
  18. Schwartz D., Veis A. Characterization of basement membrane collagen of bovine anterior lens capsule via segment-long-spacing crystallites and the specific cleavage of the collagen by pepsin. FEBS Lett. 1978 Jan 15;85(2):326–332. doi: 10.1016/0014-5793(78)80484-0. [DOI] [PubMed] [Google Scholar]
  19. Sellers A., Reynolds J. J., Meikle M. C. Neutral metallo-proteinases of rabbit bone. Separation in latent forms of distinct enzymes that when activated degrade collagen, gelatin and proteoglycans. Biochem J. 1978 May 1;171(2):493–496. doi: 10.1042/bj1710493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Timpl R., Rohde H., Robey P. G., Rennard S. I., Foidart J. M., Martin G. R. Laminin--a glycoprotein from basement membranes. J Biol Chem. 1979 Oct 10;254(19):9933–9937. [PubMed] [Google Scholar]
  21. Uitto V. J., Schwartz D., Veis A. Degradation of basement-membrane collagen by neutral proteases from human leukocytes. Eur J Biochem. 1980 Apr;105(2):409–417. doi: 10.1111/j.1432-1033.1980.tb04515.x. [DOI] [PubMed] [Google Scholar]
  22. Vuento M., Vaheri A. Dissociation of fibronectin from gelatin-agarose by amino compounds. Biochem J. 1978 Oct 1;175(1):333–336. doi: 10.1042/bj1750333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Woolley D. E., Glanville R. W., Roberts D. R., Evanson J. M. Purification, characterization and inhibition of human skin collagenase. Biochem J. 1978 Feb 1;169(2):265–276. doi: 10.1042/bj1690265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yamada K. M., Olden K. Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978 Sep 21;275(5677):179–184. doi: 10.1038/275179a0. [DOI] [PubMed] [Google Scholar]

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