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Comparative kinetics of production of CAEV-pBSCA virus from human (TE671 and THP-1) and goat (TIGEF) cells. pBSCA plasmid DNA containing CAEV-CO genome was introduced by transfection into human (TE671 and THP-1) and goat (TIGEF) cell lines. Culture medium of transfected cells was harvested on days 2, 4, 6, 8, and 10 posttransfection, clarified by filtration through a 0.45-μm-pore-size membrane, and titrated for infectious virus in GSM cells as described in Materials and Methods.
