Table 2.

Serum and blood chemistry of mice during the 9‐month study

	WT	Ctns −/−	Ctns −/− Mc4r −/−	WT	Ctns −/−	Ctns −/− Mc4r −/−	WT	Ctns −/−	Ctns −/− Mc4r −/−
	1‐month‐old (n = 8)	1‐month‐old (n = 8)	1‐month‐old (n = 8)	4‐month‐old (n = 8)	4‐month‐old (n = 8)	4‐month‐old (n = 8)	9‐month‐old (n = 8)	9‐month‐old (n = 8)	9‐month‐old (n = 8)
BUN (mg/dL)	29.6 ± 3.7	28.7 ± 2.2	31.7 ± 3.2	30.4 ± 3.6	32.7 ± 3.6	32.6 ± 3.5	30.4 ± 3.2	67.8 ± 9.5 a	72.3 ± 8.6 a
Creatinine (mg/dL)	0.08 ± 0.02	0.10 ± 0.02	0.07 ± 0.02	0.07 ± 0.02	0.12 ± 0.02	0.09 ± 0.02	0.08 ± 0.01	0.016 ± 0.02 a	0.15 ± 0.02 a
Bicarbonate (mg/dL)	27.8 ± 0.4	26.8 ± 1.3	27.9 ± 1.6	27.4 ± 0.7	26.8 ± 0.7	27.7 ± 0.5	27.9 ± 0.9	27.3 ± 1.1	27.6 ± 0.8
Calcium (mg/dL)	9.87 ± 0.15	9.58 ± 0.21	9.49 ± 0.27	9.76 ± 0.27	9.48 ± 0.13	9.85 ± 0.27	9.49 ± 0.33	9.54 ± 0.28	9.39 ± 0.75
Phosphorus (mg/dL)	9.42 ± 0.27	9.65 ± 0.37	9.59 ± 0.43	9.59 ± 0.52	9.07 ± 0.54	8.38 ± 0.48	8.87 ± 0.25	7.37 ± 0.21 b	7.39 ± 0.55 b
Intact PTH (pg/mL)	94.2 ± 13.7	116.5 ± 22.6	14.7 ± 12.7	109.6 ± 25.7	143.9 ± 18.3	135.2 ± 26.3	128.4 ± 25.1	274.1 ± 24.6 a	225.2 ± 21.8 a
FGF23 (pg/mL)	87.1 ± 21.6	79.4 ± 14.8	72.4 ± 6.9	97.2 ± 8.4	106.3 ± 12.7	86.9 ± 11.6	95.7 ± 16.4	221.6 ± 17.8 a	275.4 ± 26.3 a

All mice were fed ad libitum. Data are expressed as mean ± SEM. Results of Ctns ^−/− mice and Ctns ^−/− Mc4r ^−/− mice were compared with age‐matched WT mice.

BUN, blood urinary nitrogen; FGF23, fibroblast growth factor 23; PTH, parathyroid hormone.

^a P < 0.05, significantly higher in Ctns ^−/− mice or Ctns ^−/− Mc4r ^−/− mice than WT mice.

^b P < 0.05, significantly lower in Ctns ^−/− mice or Ctns ^−/− Mc4r ^−/− mice than WT mice.