Table 3.

Serum and blood chemistry of mice

	WT + Vehicle (n = 9)	WT + PLA (n = 9)	Ctns −/−  + Vehicle (n = 9)	Ctns −/−  + PLA (n = 9)
BUN (mg/dL)	29.5 ± 4.6	32.4 ± 3.1	59.8 ± 6.7 a	74.5 ± 9.4 a
Creatinine (mg/dL)	0.09 ± 0.02	0.12 ± 0.04	0.19 ± 0.02 a	0.21 ± 0.04 a
Bicarbonate (mg/dL)	28.2 ± 2.2	27.6 ± 2.1	27.3 ± 1.8	27.3 ± 1.1
Calcium (mg/dL)	9.36 ± 0.13	9.54 ± 0.25	9.75 ± 0.21	9.48 ± 0.27
Phosphorus (mg/dL)	8.97 ± 0.21	9.05 ± 0.31	7.76 ± 0.21 b	7.87 ± 0.21 b
Intact PTH (pg/mL)	109.6 ± 21.5	143.1 ± 22.6	435.1 ± 37.8 a	419.5 ± 23.6 a
FGF23 (pg/mL)	132.1 ± 12.8	105.2 ± 18.2	265.7 ± 34.1 a	245.3 ± 31.4 a
Leptin (ng/dL)	2.61 ± 0.31	2.83 ± 0.47	4.57 ± 0.36 a	5.17 ± 0.29 a

Twelve‐month‐old Ctns ^−/− mice and WT mice were treated with PLA (7 mg/kg/day, IP) or vehicle (normal saline) for 28 days. Four groups of mice were included: WT + vehicle, WT + PLA, Ctns ^−/−  + vehicle and Ctns ^−/−  + PLA. Ctns ^−/−  + vehicle mice were fed ad libitum whereas WT + vehicle, WT + PLA and Ctns ^−/−  + PLA mice were pair‐fed to that of Ctns ^−/−  + vehicle mice. Data are expressed as mean ± SEM. Data are expressed as mean ± SEM. Results of Ctns ^−/−  + vehicle mice were compared with that of WT + vehicle mice whereas results of Ctns ^−/−  + PLA mice were compared with that of WT + PLA mice.

^a P < 0.05, significantly higher in Ctns ^−/− mice than WT mice.

^b P < 0.05, significantly lower in Ctns ^−/− mice than WT mice.