TABLE 2.

Amplification of cloned and plant-adapted chimeric TYMV in plants and protoplasts

TYMV genome	Relative amplification of cloned progenitor genome in protoplastsa	Amplification of plant-adapted progeny virus				Amplification of cloned progeny genomes
		In plantsb		In protoplastsc		In plantsd		In protoplastse
		Rel. accum. of coat protein	Rel. accum. of genomic RNA	Rel. accum. of coat protein	Rel. accum. of genomic RNA	Rel. accum. of coat protein	Rel. accum. of genomic RNA	Rel. accum. of coat protein	Rel. accum. of genomic RNA
WT (transcript)	1.00					1.00 ± 0.07	1.00 ± 0.04	1.00 ± 0.04	1.00 ± 0.03
WT (virion)		1.00 ± 0.16	1.00 ± 0.05	1.00 ± 0.06	1.00 ± 0.02
-TYV	0.08
-XX		1.01 ± 0.10	0.88 ± 0.07
-XX1						0.58 ± 0.07	0.55 ± 0.08	0.54 ± 0.03	0.35 ± 0.06
-TYV-BP	0.12
-YY		0.97 ± 0.06	0.68 ± 0.10
-YY1							0.54 ± 0.04	0.43 ± 0.03	0.29 ± 0.06
-YY1/U6150						0.67 ± 0.08		0.53 ± 0.03	0.42 ± 0.07
-ELV	0.14
-H		0.69 ± 0.03	0.86 ± 0.11
-H1						0.52 ± 0.03	0.65 ± 0.09	0.25 ± 0.08	0.20 ± 0.04
-EMV	0.22
-E				0.35 ± 0.02	0.26 ± 0.08
-Met	0.47
-M				0.44 ± 0.05	0.47 ± 0.08

^aRelative levels of genomic RNA detected in extracts made from turnip protoplasts 40 h postinfection determined by Northern blotting; data taken from references 7, 9, and 18. 

^bRelative accumulations (Rel. accum.) in Chinese cabbage plants, cv. Spring A-1, inoculated with 1 μg of virion RNA. Viral coat protein levels were determined by DAS-ELISA; accumulation after inoculation with TYMC virion RNA was 3.34 ± 0.16 mg of viral coat protein per g of fresh leaf tissue. Genomic RNA levels were determined via Northern blots. Averages from at least three analyses of pooled leaves from five to six plants harvested 15 days postinfection are presented. 

^cInoculation of 0.5 × 10⁶ to 1 × 10⁶ Chinese cabbage cv. W-R60 Green protoplasts with 1 μg of virion RNA. Coat protein levels and genomic RNA levels were determined by Western and Northern blotting, respectively; averages are from at least three independent inoculation experiments. 

^dChinese cabbage plants, cv. Spring A-1, inoculated with 5 μg of 5′-capped genomic transcripts. Viral coat protein levels were determined by DAS-ELISA; accumulation after inoculation with TYMC transcript RNA was 2.74 ± 0.19 mg of coat protein per g of fresh leaf tissue. Genomic RNA levels were determined via Northern blots. Averages from at least three analyses of pooled leaves from five to six plants harvested 15 days postinfection are presented. 

^eInoculation of 0.5 × 10⁶ to 1 × 10⁶ Chinese cabbage cv. W-R60 Green protoplasts with 5 μg of 5′-capped genomic transcripts. Extracts were made at 40 h postinfection, and coat protein and genomic RNA levels were determined by Western and Northern blotting, respectively; averages are from at least three independent inoculation experiments.