Fig. 4.

S100A8/A9 blockade significantly impacts the abundance of angiogenesis-related proteins in the post-ischemic myocardium. Differential proteomic analysis of infarcted LV from the PBS-treated (n = 4; 2 females and 2 males) and the ABR-treated (n = 5; 3 females and 2 males) MI groups. (A) Volcano plot depicting the distribution of differentially abundant proteins, separated by fold change and adjusted p-value. The green dots are significantly down-regulated proteins (ratio of medians MI + ABR/MI < 0.8, p < 0.05) and the pink dots are significantly up-regulated (ratio of medians MI + ABR/MI > 1.25, p < 0.05). Proteins associated with angiogenesis are marked by purple circles. Proteins upregulated or down-regulated by more than fourfold are annotated with the gene name. The p-values were obtained using ANOVA followed by the Benjamini‐Hochberg correction for false discovery rate. (B) Heatmap representation of differentially expressed proteins involved in angiogenesis-linked biological processes between the PBS-treated MI (n = 4) and sham (n = 3) groups, and between the ABR- and PBS-treated groups, respectively. The colour scale indicates the regulation level between the groups (red represents up-regulation and blue down-regulation). DEPs were considered to be significant at an adjusted p-value < 0.05. *p < 0.05, **p < 0.01, ***p < 0.001