FIG. 6.

Activation of NF-IL6 in RSV-CM-stimulated A549 cells. (A) Nuclear extracts were prepared from A549 cells at 0, 2, 4, 8, and 24 h poststimulation with either C-CM or RSV-CM. Equal quantities of nuclear protein were mixed with ³²P-labeled, double-stranded oligonucleotides specific for the NF-IL6 binding sequence and were resolved by PAGE. Bound complexes were visualized by autoradiography. (B) Competition experiments were performed on extracts prepared 24 h poststimulation with RSV-CM. NF-IL-6 binding to labeled probe was not competed out with a 10-fold excess of unlabeled NF-κB probe but was completely blocked with a 10-fold excess of unlabeled NF-IL6 probe.