Reagent	Final concentration	Quantity or Volume
1× Murashige and Skoog medium (without N, P, and Fe)	0.1×	10 mL
Sucrose	0.5% (w/v)	0.5 g
100 mM ammonium succinate stock solution	500 µM	0.5 mL
1 M potassium dihydrogen phosphate stock solution	125 µM	12.5 µL
170 mM MES stock solution	3.5 mM	2.1 mL
10 mM iron (II) sulfate stock solution (add after autoclave)	10 µM	100 µL
Ultrapure water (Milli-Q)	n/a	Up to 100 mL
Total	n/a	100 mL