FIGURE 2.

Rab10 deficiency in NAc regions inhibited cocaine-induced behavioral sensitization by increasing the plasma membrane expression of GABA_BR. (A) Experimental timeline of the cocaine-induced behavioral sensitization in Rab10 deficiency rats. (B) Measurement of the total traveling distance in the open-field test (n = 6). Data were presented as the means ± SEM. **p < 0.01 compared with the AAV + saline group; ##p < 0.01 and ###p < 0.001 compared with AAV + cocaine-treated group on day 1; ++p < 0.01 compared with the AAV + cocaine group. (C) Quantification of the relative protein level of GABA_B1R, GABA_B2R, Rab10, CaMKIIa, and pCaMKIIa versus pan-Cadherin and normalization versus AAV + saline group (n = 3). Data were presented as the means ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the AAV + saline group; +++p < 0.001 compared with the AAV + cocaine group; ###p < 0.001 compared with AAV-Rab10-siRNA + saline groups; n.s., no significance. (D) Representative plasma membrane protein Western blot of GABA_B1R, GABA_B2R, CaMKIIa, pCaMKIIa, and Rab10 obtained at 5 days post-treatment.