FIGURE 3.

Cocaine treatment inhibited plasma membrane expression of Rab10 in NAc GAD ₆₇ ⁺ cells. (A) NAc GAD ₆₇ ⁺ cells were stained with antibodies against Rab10 (green) and GFP (gray). Scale bar, 10 μm. Cells were treated with saline, baclofen, or cocaine. (B) Data from (A) were presented as the means ± SEM (n = 3). ###p < 0.001 and **p < 0.01 compared with the saline group. (C) Dot plot analysis of GAD ₆₇ ⁺ neurons from NAc. (D) Relative fluorescence intensity of Rab10⁺/GAD ₆₇ ⁺ (n = 3). Data were presented as the means ± SEM. *p < 0.05 compared with the control group. (E) Representative plasma membrane protein Western blot of Rab10 obtained after treatment with saline, baclofen, or cocaine. (F) Quantification of the relative protein level of Rab10 versus pan-Cadherin and normalization versus saline (n = 3). ##p < 0.01 and *p < 0.05 compared with the saline group.