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. 2024 Nov 27;15:1496657. doi: 10.3389/fphar.2024.1496657

FIGURE 4.

FIGURE 4

Differential GABABR plasma membrane expression in NAc neurons from Rab10 deficiency mice under cocaine treatment. (A) Representative plasma membrane protein Western blot of Rab10, GABAB1R, and GABAB2R in NAc cells from wild-type (WT, Nestin+; Rab10 +/+) or Rab10-deficient (WT, Nestin+; Rab10 F/F ) mice after saline or cocaine treatment. Data were presented as the means ± SEM. (B) Quantification of the relative protein level of GABABR and Rab10 versus pan-Cadherin (n = 3). Data were presented as the means ± SEM. **p < 0.01 compared with the wild-type (WT, Nestin+; Rab10 +/+) with saline treatment; ##p < 0.01 and #p < 0.05 compared with wild-type (WT, Nestin+; Rab10 +/+) with cocaine treatment. (C) NAc cells were stained with antibodies against GABAB1R (green), GABAB2R (red), and DAPI (blue). Scale bar, 10 μm. (D) Data were presented as the means ± SEM (n = 3). ***p < 0.001; n.s., no significance. (E) NAc neurons were stained against surface GABAB1R (red) and internalized GABAB1R (green). Scale bar, 10 μm. (F) NAc neurons transfected with GABAB1R-pHluorin were stimulated with ∼470 nm laser, followed by time-lapse imaging. White dashed line: cell membrane. Scale bar, 10 μm. (G) Normalized fluorescence intensity of GABAB1R-pHluorin.