Abstract
A hypothesis of Mason & Kodicek [(1970) Biochem. J. 120, 515-521] that esterified nicotinic acid in niacytin from cereals is a precursor for trigonelline was investigated in rats. Single oral doses of niacytin resulted in the excretion of trigonelline in urine but only in rats that were niacin-deficient and were fed a cereal diet. These animals were found to have an abnormally permeable intestine, which allowed the uptake of molecules not usually absorbed. Orally administered synthetic [14C]nicotinoyl[3H]methylcellulose was shown to be absorbed by niacin-deficient rats on a cereal diet and [14C]trigonelline was excreted in urine. These data indicate that dietary cereal induces a permeability defect in the intestinal mucosa of niacin-deficient rats, which allows the uptake of macromolecular niacytin. The nicotinoyl pyridine nitrogen atom is then methylated and slow hydrolysis releases trigonelline from the macromolecule.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cobden I., Rothwell J., Axon A. T. Intestinal permeability and screening tests for coeliac disease. Gut. 1980 Jun;21(6):512–518. doi: 10.1136/gut.21.6.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCKENNIS H., Jr, BOWMAN E. R., HORVATH A., BEDERKA J. P., Jr METABOLIC RELEASE OF METHYL GROUPS FROM A SERIES OF N-METHYLPYRIDINIUM COMPOUNDS. Nature. 1964 May 16;202:699–700. doi: 10.1038/202699a0. [DOI] [PubMed] [Google Scholar]
- Mason J. B., Gibson N., Kodicek E. The chemical nature of the bound nicotinic acid of wheat bran: studies of nicotinic acid-containing macromolecules. Br J Nutr. 1973 Sep;30(2):297–311. doi: 10.1079/bjn19730035. [DOI] [PubMed] [Google Scholar]
- Mason J. B., Kodicek E. The metabolism of niacytin in the rat. Trigonelline as a major metabolite of niacytin in the urine. Biochem J. 1970 Dec;120(3):515–521. doi: 10.1042/bj1200515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakashima Y., Sanada H., Utsuki Y., Kawada S. Effect of nicotinic acid on catecholamine synthesis in rat brain. J Nutr Sci Vitaminol (Tokyo) 1978;24(2):67–76. doi: 10.3177/jnsv.24.67. [DOI] [PubMed] [Google Scholar]
- ROE J. H. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed] [Google Scholar]
- Sandhu J. S., Fraser D. R. Measurement of niacin metabolites in urine by high pressure liquid chromatography. A simple, sensitive assay of niacin nutritional status. Int J Vitam Nutr Res. 1981;51(2):139–144. [PubMed] [Google Scholar]