TABLE 1.
Summary of the quantitative analysis of proteins implicated in (A) glutamate function (B) and neuropathological processes within the entorhinal cortex. Significant results are bolded.
| A | Glutamate‐related proteins—entorhinal cortex | |||
|---|---|---|---|---|
| Protein of interest | Age (months) | Main effect: drinking history | Interaction effect: sex by drinking history | Significant group comparisons |
| mGlu1 | 6M | Not significant |
Significant F(1,34) = 7.71, p = 0.009, η 2 = 0.185 |
Female EtOH = Female H2O (p = 0.342) Male EtOH > Male H 2 O (p = 0.006) Female EtOH < Male EtOH (p < 0.001) Female H2O = Male H2O (p = 1.000) |
| 9M |
Not significant p = 0.255, η 2 = 0.039 |
Not significant F(1,33) = 3.74, p = 0.062, η 2 = 0.102 |
None | |
| 12M |
Not significant p = 0.960, η 2 = 0.000 |
Not significant F(1,31) = 1.11, p = 0.301, η 2 = 0.034 |
None | |
| mGlu5 dimer | 6M |
Not significant p = 0.737, η 2 = 0.003 |
Not significant F(1,39) = 0.01, p = 0.927, η 2 = 0.000 |
None |
| 9M |
Not significant p = 0.376, η 2 = 0.024 |
Not significant F(1,33) = 1.04, p = 0.315, η 2 = 0.031 |
None | |
| 12M |
Not significant p = 0.262, η 2 = 0.043 |
Not significant F(1,29 = 0.14, p = 0.716, η 2 = 0.005 |
None | |
| mGlu5 monomer | 6M |
Not significant p = 0.771, η 2 = 0.002 |
Not significant F(1,35) = 1.99, p = 0.167, η 2 = 0.054 |
None |
| 9M |
Significant F(1,34) = 4.73, p = 0.037, η 2 = 0.122 |
Not significant F(1,34) = 1.02, p = 0.319, η 2 = 0.029 |
EtOH < H 2 O (p = 0.037) | |
| 12M |
Significant F(1,27) = 12.59, p = 0.001, η 2 = 0.318 |
Not significant F(1,27) = 2.04, p = 0.164, η 2 = 0.070 |
EtOH > H 2 O (p = 0.001) | |
| GluN1 | 6M |
Not significant p = 0.065, η 2 = 0.087 |
Not significant F(1,38) = 0.63, p = 0.434, η 2 = 0.016 |
None |
| 9M |
Not significant p = 0.967, η 2 = 0.000 |
Not significant F(1,38) = 0.07, p = 0.797, η 2 = 0.002 |
None | |
| 12M |
Not significant p = 0.245, η 2 = 0.050 |
Significant F(1,27) = 1.91, p = 0.010, η 2 = 0.220 |
Female EtOH = Female H2O (p = 0.317) Male EtOH > Male H 2 O (p = 0.005) Female EtOH < Male EtOH (p = 0.001) Female H2O = Male H2O (p = 1.000) |
|
| GluN2B | 6M |
Not significant p = 0.075, η 2 = 0.003 |
Not significant F(1,40) = 0.68, p = 0.416, η 2 = 0.017 |
None |
| 9M |
Significant F(1,34) = 7.71, p = 0.002, η 2 = 0.244 |
Significant F(1,34) = 7.71, p = 0.009, η 2 = 0.185 |
Female EtOH = Female H2O (p = 0.681) Male EtOH < Male H 2 O (p < 0.001) Female EtOH > Male EtOH (p < 0.001) Female H2O = Male H2O (p = 1.000) |
|
| 12M |
Not significant p = 0.123, η 2 = 0.092 |
Significant F(1,25) = 11.24, p = 0.003, η 2 = 0.310 |
Female EtOH = Female H2O (p = 0.270) Male EtOH > Male H 2 O (p < 0.001) Female EtOH < Male EtOH (p < 0.001) Female H2O = Male H2O (p = 1.000) |
|
| Homer 1b/c | 6M |
Not significant p = 0.758, η 2 = 0.003 |
Not significant F(1,37) = 0.16, p = 0.693, η 2 = 0.004 |
None |
| 9M |
Not significant p = 0.596, η 2 = 0.009 |
Not significant F(1,32) = 0.47, p = 0.499, η 2 = 0.014 |
None | |
| 12M |
Not significant p = 0.191, η 2 = 0.053 |
Not significant F(1,32) = 0.53, p = 0.474, η 2 = 0.016 |
None | |
| Homer 2a/b | 6M |
Not significant p = 0.560, η 2 = 0.010 |
Not significant F(1,36) = 0.08, p = 0.738, η 2 = 0.002 |
None |
| 9M |
Not significant p = 0.642, η 2 = 0.007 |
Not significant F(1,32) = 1.76, p = 0.195, η 2 = 0.052 |
None | |
| 12M |
Significant F(1,25) = 4.59, p = 0.042, η 2 = 0.155 |
Not significant F(1,25) = 1.58, p = 0.221, η 2 = 0.059 |
EtOH > H 2 O (p = 0.042) | |
| ERK | 6M |
Not significant p = 0.069, η 2 = 0.080 |
Not significant F(1,40) = 0.71, p = 0.406, η 2 = 0.017 |
None |
| 9M |
Not significant p = 0.600, η 2 = 0.008 |
Not significant F(1,33) = 0.93, p = 0.342, η 2 = 0.027 |
None | |
| 12M |
Significant F(1,28) = 8.59, p = 0.007, η 2 = 0.235 |
Significant F(1,28) = 15.52, p < 0.001, η 2 = 0.357 |
Female EtOH < Female H 2 O (p < 0.001) Male EtOH = Male H2O (p = 0.400) Female EtOH < Male EtOH (p < 0.001) Female H2O = Male H2O (p = 1.000) |
|
| pERK | 6M |
Not significant p = 0.281, η 2 = 0.040 |
Not significant F(1,29) = 0.40, p = 0.531, η 2 = 0.014 |
None |
| 9M |
Significant F(1,31) = 5.70, p = 0.023, η 2 = 0.155 |
Significant F(1,31) = 7.30, p = 0.011, η 2 = 0.191 |
Female EtOH = Female H2O (p = 0.827) Male EtOH > Male H 2 O (p = 0.001) Female EtOH < Male EtOH (p < 0.001) Female H2O = Male H2O (p = 1.000) |
|
| 12M |
Not significant p = 0.717, η 2 = 0.005 |
Not significant F(1,29) = 1.75, p = 0.196, η 2 = 0.057 |
None | |
| B | Neuropathological protein expression—entorhinal cortex | |||
|---|---|---|---|---|
| Protein of interest | Age (months) | Main effect: drinking history | Interaction effect: sex by drinking history | Significant group comparisons |
| Tau | 6M |
Not significant p = 0.288, η 2 = 0.032 |
Not significant F(1,35) = 1.15, p = 0.291, η 2 = 0.032 |
None |
| 9M |
Not significant p = 0.550, η 2 = 0.011 |
Not significant F(1,33) = 0.55, p = 0.463, η 2 = 0.016 |
None | |
| 12M |
Not significant p = 0.053, η 2 = 0.132 |
Not significant F(1,27) = 0.03, p = 0.877, η 2 = 0.001 |
None | |
| pThr(217) Tau | 6M |
Not significant p = 0.840, η 2 = 0.001 |
Not significant F(1,34) = 0.04, p = 0.838, η 2 = 0.001 |
None |
| 9M |
Significant F(1,33) = 6.75, p = 0.014, η 2 = 0.170 |
Significant F(1,33) = 5.96, p = 0.020, η 2 = 0.153 |
Female EtOH = Female H2O (p = 0.908) Male EtOH > Male H 2 O (p = 0.002) Female EtOH < Male EtOH (p < 0.001) Female H2O = Male H2O (p = 1.000) |
|
| 12M |
Significant F(1,28) = 16.51, p < 0.001, η 2 = 0.371 |
Not significant F(1,28) = 2.26, p = 0.144, η 2 = 0.075 |
EtOH > H 2 O (p < 0.001) | |
| pSer(396) Tau | 6M |
Not significant p = 0.934, η 2 = 0.000 |
Not significant F(1,37) = 1.03, p = 0.317, η 2 = 0.027 |
None |
| 9M |
Significant F(1,33) = 15.17, p < 0.001, η 2 = 0.315 |
Not significant F(1,33) = 1.99, p = 0.168, η 2 = 0.057 |
EtOH < H 2 O (p < 0.001) | |
| 12M |
Not significant p = 0.349, η 2 = 0.030 |
Not significant F(1,23) = 3.43, p = 0.077, η 2 = 0.111 |
None | |
| BACE 56 kDa | 6M |
Significant F(1,32) = 5.27, p = 0.028, η 2 = 0.141 |
Not significant F(1,32) = 0.82, p = 0.371, η 2 = 0.025 |
EtOH < H 2 O (p = 0.028) |
| 9M |
Not significant p = 0.609, η 2 = 0.008 |
Not significant F(1,32) = 0.174, p = 0.679, η 2 = 0.005 |
None | |
| 12M |
Not significant p = 0.172, η 2 = 0.061 |
Not significant F(1,26) = 2.32, p = 0.140, η 2 = 0.071 |
None | |
| BACE 70 kDa | 6M |
Significant F(1,36) = 17.63, p < 0.001, η 2 = 0.329 |
Not significant F(1,36) = 0.12, p = 0.731, η 2 = 0.003 |
EtOH < H 2 O (p < 0.001) |
| 9M |
Not significant p = 0.606, η 2 = 0.009 |
Not significant F(1,31) = 0.52, p = 0.476, η 2 = 0.016 |
None | |
| 12M |
Significant F(1,28) = 5.53, p = 0.026, η 2 = 0.157 |
Not significant F(1,28) = 0.79, p = 0.380, η 2 = 0.023 |
EtOH > H 2 O (p = 0.026) | |
| APP | 6M |
Not significant p = 0.191, η 2 = 0.044 |
Not significant F(1,39) = 0.23, p = 0.638, η 2 = 0.006 |
None |
| 9M |
Not significant p = 0.476, η 2 = 0.014 |
Not significant F(1,36) = 0.00, p = 0.958, η 2 = 0.000 |
None | |
| 12M |
Significant F(1,32) = 15.82, p < 0.001, η 2 = 0.331 |
Not significant F(1,32) = 0.47, p = 0.500, η 2 = 0.014 |
EtOH > H 2 O (p < 0.001) | |
| Aβ | 6M |
Not significant p = 0.081, η 2 = 0.089 |
Not significant F(1,33) = 2.94, p = 0.096, η 2 = 0.082 |
None |
| 9M |
Significant F(1,37) = 10.09, p = 0.003, η 2 = 0.214 |
Not significant F(1,37) = 0.04, p = 0.836, η 2 = 0.001 |
EtOH < H 2 O (p = 0.003) | |
| 12M |
Not significant p = 0.328, η 2 = 0.029 |
Not significant F(1,33) = 3.70, p = 0.063, η 2 = 0.101 |
None | |