Table 2.
Comparison of RT-PCR kits specifications.
| Parameter | ONT-recommended SSIII | Alternative SSIV | Alternative LS |
|---|---|---|---|
| RT-PCR Kit | SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) | SuperScript IV One-Step RT-PCR System (Invitrogen) | LunaScript Multiplex One-Step RT-PCR Kit (New England Biolabs) |
| One-step RT-PCR | Yes | Yes | Yes |
| Reverse Transcriptase | SuperScript III | SuperScript IV | Luna Warm Start |
| Recommended RT Time | 15-30 min | 10 min | 10 min |
| DNA Polymerase | Platinum Taq | Platinum SuperFi | Q5 Hot Start High-Fidelity |
| Fidelity (vs. Taq) | 1X | 300X | 280X |
| Hot-start Temperature | 94°C | 98°C | 98°C |
| GC-Rich PCR Performance | High | High | High |
| Optimal amplicon length | 200 - 4,500 bp | Up to 13.8 kb | 100 - 1,500 bp |
| Price per sample | $8.62 | $9.71 | $3.86 |