Table 4.

Summary of purification quality before NGS library preparation.

Sample	Quantity - Qubit 1x HS, ng/µl 1					Quality - Nanodrop, A260/A280					Average Length - Tape Station, bp
	Unpurified	Kit 1	Kit 2	Kit 3	Kit 4	Unpurified	Kit 1	Kit 2	Kit 3	Kit 4	Unpurified	Kit 1	Kit 2	Kit 3	Kit 4
H5N1	112	100.8	47.8	96	23.5	1.79	1.86	1.80	1.86	1.81	1356	1409	1397	1413	1796
H7N3	120	127.2	40.6	116	17.9	1.79	1.88	1.80	1.88	1.83	1330	1383	1413	1406	1725
H7N7	82.4	70.2	36.4	64.4	12.8	1.81	1.85	1.80	1.85	1.79	1006	1087	1116	1087	1533
H1N1	106	85.8	39.6	81.2	18.6	1.80	1.85	1.79	1.86	1.80	1278	1378	1377	1298	1704
H6N2	106	88.2	41.1	91.4	19.0	1.79	1.85	1.80	1.85	1.79	1209	1349	1320	1256	1712
H8N4	112	91.8	39.3	108	20.6	1.80	1.85	1.80	1.86	1.81	1468	1551	1496	1503	1929
Average	313.3	94	40.8	92.8	18.8	1.80	1.86	1.80	1.86	1.80	1274.5	1359.5	1353.2	1327.2	1733.2

¹Concentrations after normalization to equal volume.