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RNA blot hybridization analysis. Total cytoplasmic RNA isolated from ANV-infected cells and uninfected cells at the indicated hours p.i. were resolved in a 1.0% agarose gel which contained 0.1% sodium dodecyl sulfate, transferred to a nylon membrane, and probed with DIG-labeled ANV probes (7FB-7RB, 5′ end; 1F-1R, 3′ end). The positions of the 28S and 18S rRNAs are indicated on the left; those of genonic (7.5-kb) and subgenonic (3.0-kb) RNAs are indicated on the right. cont, control.
