TABLE 1.
PCR primers used to generate PCR clones of ANV cDNA and the DIG-labeled probes for Northern blot hybridization
| Primer | Location (nt) | Sequencea |
|---|---|---|
| Sense | ||
| BT 7 | 1–22 | cgg cgg gat cct aat acg act cac tat agC CGAATA GAT GGG ATG GCT TCG |
| S 1 | 539–561 | GAG AAC GAG AGA ACC AAA CAC GG |
| S 2 | 583–604 | GTG CCT CTG GAT CTT TCT TGC G |
| 7FB | 1897–1916 | GGA CGG GAG GAT GCT TGG TG |
| 1F | 6596–6615 | CTA GAC CTC CGG CGC TTT CA |
| Antisense | ||
| A 2 | 382–356 | CAG GAG CGT GCG GGT GAG AAC AAC |
| A 1 | 473–450 | CTT TCA CTT TTT CAC GCG CCA GGT |
| P-12R | 661–638 | AAC ATC GAC AAG GGT ACA CGC TGC |
| 7RB | 2198–2179 | CAC GAA CGG TCC TCT TAG TC |
| 1R | 6879–6859 | CGG GAG AAC CAA CTT TAG CGC |
a
Uppercase letters, primer sequence corresponding to (5′ primer) or complementary to (3′ primer) the ANV genonic sequence; lowercase letters, sequence used to introduce restriction site and T7 promoter.