Table 4.
Effect of B. subtilis on P and sugar content in shoots and roots and gene expression
| Cultivar | P level (mg L− 1) |
B. subtilis
inoculation |
P content (mg plant− 1) |
PAE (mg P uptake g− 1 root DM) |
Sugar content in roots (102 mg plant− 1) |
P transport genes in leaves (relative expression) |
YwkB gene expression in roots (relative expression) |
||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Shoots | Roots | Shoot | Roots | StPHT1;1 | StPHT1;7 | StPHT2;1 | |||||
| Lady Claire | 0.5 | -B | 12.77 ± 1.24 | 7.28 ± 0.57 | 2.78 ± 0.22 | 2.25 ± 0.36 | 0.35 ± 0.05a | 1.69 ± 0.73 | 1.08 ± 0.28 | 1.09 ± 0.22 | 1.38 ± 0.75 |
| +B | 15.86 ± 1.92 | 8.24 ± 0.76 | 3.07 ± 0.40 | 2.95 ± 0.34 | 0.46 ± 0.05b | 3.68 ± 1.29 | 1.52 ± 0.38 | 1.96 ± 0.38 | 1.93 ± 0.90 | ||
| 2 | -B | 32.80 ± 3.04 | 9.55 ± 0.65 | 4.56 ± 0.58 | 5.89 ± 0.24 | 0.85 ± 0.10 | 3.12 ± 1.38 | 1.74 ± 0.30 | 1.88 ± 0.60 | 0.61 ± 0.03b | |
| +B | 36.93 ± 1.98 | 9.87 ± 0.45 | 4.77 ± 0.25 | 6.26 ± 0.84 | 1.03 ± 0.09 | 2.55 ± 1.17 | 1.27 ± 0.27 | 1.39 ± 0.44 | 1.31 ± 0.01a | ||
| Milva | 0.5 | -B | 14.16 ± 0.65 | 7.90 ± 0.28 | 2.75 ± 0.14 | 1.41 ± 0.09 | 0.53 ± 0.09b | 1.40 ± 0.47b | 0.94 ± 0.17 | 0.85 ± 0.16 | 1.02 ± 0.22 |
| +B | 15.08 ± 0.96 | 8.25 ± 0.43 | 2.77 ± 0.16 | 1.53 ± 0.09 | 0.70 ± 0.09a | 4.20 ± 1.08a | 1.56 ± 0.17 | 1.83 ± 0.41 | 1.31 ± 0.15 | ||
| 2 | -B | 51.81 ± 3.57 | 9.56 ± 0.50 | 6.56 ± 0.70 | 6.74 ± 0.68 | 1.15 ± 0.07 | 1.56 ± 0.92 | 0.94 ± 0.31 | 1.25 ± 0.34 | 1.23 ± 0.85 | |
| +B | 51.81 ± 3.15 | 9.75 ± 0.44 | 9.34 ± 0.31 | 7.64 ± 0.88 | 1.12 ± 0.07 | 0.57 ± 0.24 | 0.86 ± 0.17 | 0.60 ± 0.21 | 1.42 ± 0.53 | ||
Transcription levels are presented as relative values to a mix of cDNA from all treatments. Means ± SE (n = 5) followed by different lowercase letters in the same column are significantly different between with and without B. subtilis inoculation at the same P level and cultivar. PAE = P acquisition efficiency, B = B. subtilis, no indication = not significantly different.