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. 2024 Apr 2;15(4):564–584. doi: 10.1080/21501203.2024.2323713

Table 3.

Enzymatic and whole-cell immobilisation for continuous FOS generation.

SN Microorganism Sucrose
(w/v)
Optimum
Carrier Yield
(% w/w sucrose)
FOS products Recycling Reference
Temp
(°C)
pH
1 Aspergillus tritici BGPUP6 Inulin
(7.0%)
- - Halloysite nanoclay 99.56% FOS GF4, GF3, GF2, and FOSs DP5–9 3 cycles Singh and Singh 2022a, 2022b)
2 A. tritici Inulin
(8.0%)
60 5.0 Halloysite nanoclay 95.44% FOS GF4, GF3, and GF2 8 cycles Singh and Singh (2022b)
3 A. tritici Inulin
(10%)
65 5.0 Halloysite nanoclay using 3-amino-propyltriethoxysilane 98.42% FOS GF4, GF3, GF2 and FOSs DP 5–9 18 cycles Singh and Singh (2022c)
4 Aspergillus oryzae IPT-301   30   Polyhydroxybutyrate 55% FOS   6 cycles Araújo et al. (2022)
5 Penicillium brevicompactum FTase in Pichia pastoris GS115 (Muts) Sucrose (600 g/L) 45 6.0 Lifetech ECR8285 (methacrylate polymer) 130–170 g/L GF4, GF3, and GF2 - Fang et al. (2021)
6 Aspergillus niger ATCC 20611 Sucrose
(50%)
50 5.5 Mycelia of A. niger 51.05% FOS - 6 cycles Wang et al. (2021)
7 Schedonorus arundinaceus (P. pastoris) 600 g/L 30 5.5 Calcium-alginate 55% FOS 1-Kestotriose and 1,1-Kestotetraose 15 cycles Pérez et al. (2021)
8 Rhodotorula mucilaginosa Inulin
250 g/L
(25%)
75 8.0 Celite and chicken eggshell 9.49 g/L GF3
GF2
16 h de Araujo Ribeiro et al. (2022)
9 A. oryzae IPT-301 47 60 5.5 Silica gel - GF2 6 cycles Faria et al. (2021)
10 Aspergillus aculeatus 700 g/L 60 7.0 Fe3O4-Chitosan- magnetic nanoparticles 101.56 g/L GF3, GF2 6 cycles de Oliveira et al. (2020)
11 Aspergillus terreus 1.4–1.9
Molar
70 5.8 Sepabeads SP70, Amberlite XAD16N, and immobead 150P carriers 60% FOS yields GF5, GF4, GF3, and GF2 - Burghardt et al. (2020)
12 Aspergillus tamarii NKRC 1229 50 20 7.0 Poly-Prep chromatography column 50% GF3, GF2 10 cycles Choukade and Kango (2019)
13 Aureobasidium sp. ATCC 20524 100, 300, 600 g/L 50 5.0 Titanium oxide 60, 59, 62 GF4, GF3, and GF2 7 cycles Valdeon et al. (2019)
14 Aspergillus japonicus (fopA) 60% 62 5.0 Amberlite IRA 900 and calcium alginate 53% and 59% FOS GF4, GF3, and GF2 15 cycles Bedzo et al. (2019)
15 Xanthophyllomyces dendrorhous 600 g/L 30 5.0 Polyvinyl alcohol hydrogel 18.9% FOS yields Neokestose, 1-Kestose, neonystose, blastose 7 cycles Miguez et al. (2018)
16 Aspergillus aculeatus M105 600 65 5.0–6.0 Sodium alginate 65.47 GF4, GF3, and GF2   Huang et al. (2016)
17 A. aculeatus 600 50 5.5 Chitosan 55 GF3, GF2 50 cycles Lorenzoni et al. (2014)
18 Aspergillus flavus NFCCI 2364 60 55 5.5–6.0 Calcium alginate, chitosan 68
43
GF4, GF3, and GF2 15 cycles Ganaie et al. (2014)
19 Aureobasidium pullulans 770 50 - Calcium alginate 57 GF4, GF3, and GF2 100 days Jung et al. (2011)
20 A. japonicus 165 g/L - - Brewers spent grain, wheat straw, corn cobs, coffee husks, cork oak, loofa sponge 128.3–138.7 g/L GF4, GF3, and GF2 36 h Mussatto et al. (2009)