FIG. 2.

Characterization of full-length PERV RNA and DNA. Long PCR performed on (A) plasmid Tsukuba-1 DNA, (B) porcine DNA extracted from whole blood of one offspring in each pig family, and (C) cDNA derived from major pig organs (lanes +) and negative controls without reverse transcriptase (lanes −). A specific amplification product was obtained for cloned PERV sequence, whereas porcine DNA revealed multiple products of viral origin. RT-PCR demonstrated the existence of full-length and spliced viral RNA in all pig tissues studied.