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. 2024 Dec 12;13:RP96150. doi: 10.7554/eLife.96150

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Sequence-based reagent Primer-amel-Fwd This paper PCR primers CCCCTGGGCACTGTAAAGAAT
Sequence-based reagent Primer-amel-Rev This paper PCR primers CCAAGCATCAGAGCTTAAACTG
Sequence-based reagent Probe-amelX This paper PCR probe CCAAATAAAGTGGTTTCTCAAGT
Sequence-based reagent Probe-amelY This paper PCR probe CTTGAGAAACATCTGGGATAAAG
Commercial assay or kit ddPCR supermix for Probes (no dUTP) Biorad PCR mix
Commercial assay or kit SureSelect XT Low Input kit Agilent NGS custom RNA-baits panel
Software, algorithm Quantasoft Biorad Analysis software
Software, algorithm R CRAN https://www.r-project.org/