FIG. 4.

ORF50-cDNA clone transactivated the vIRF promoter and the DNA polymerase promoter in 293T (A) and CV-1 cells (B). The expression constructs for the ORF50-cDNA clone (solid bars) or the ORF50-genomic clone (open bars) were cotransfected with the luciferase reporter constructs driven by the vIRF promoter or the DNA polymerase promoter into 293T cells or CV-1 cells. As a negative control, a promoterless vector, pGL3.basic (A), or a DNA polymerase promoter orientation reverse construct, POL.promoter.R (B), was also included. The relative luciferase activities were normalized to the β-Gal activity. The luciferase activities are shown as percentages compared with the vIRF promoter value, which was defined as 100%. The average (with standard deviation) of duplicate experiments is represented. (C) vIRF promoter activities in different cell lines with (solid bars) or without (open bars) TPA treatment. The luciferase activities were normalized to the β-Gal activity and are shown as fold increases compared with the activity of the promoterless pGL3.basic vector.