FIG. 7.

Luciferase reporter assay of the response elements to the ORF 50 protein in the vIRF promoter region. (A) Schematic diagram of the vector (p e1b luc) and the constructs (p e1b luc-RE). The locations of RE1, RE2, and RE3 are shown. The arrows show the locations of the vIRF promoter deletion mutants. (B) The relative luciferase activity of the RE reporter constructs in response to the ORF 50 protein. The mean luciferase activities in response to the ORF 50 protein obtained from three transfections after normalization for β-Gal expression are indicated as fold increases relative to the pcDNA3.1 vector control.