TABLE 1.
Oligonucleotide primers used in site-directed mutagenesisa
| Mutant | Nucleotide sequence |
|---|---|
| E104W | 5′-ATTCGCCGCCCACGTTGTAAA-3′ |
| R111F | 5′-CCCAGTCAGAAAAGCTATTTC-3′ |
| W119D | 5′-CGCTGGCCCGTCTGTCGAAGT-3′ |
| F268R | 5′-GTAAACATTGCGAATCTCAGC-3′ |
| Y271R | 5′-CCTGAAGCTGCGAACATTGAA-3′ |
| D318N | 5′-AGTGTAAACATTAGCAAGTGT-3′ |
| T321R | 5′-CCTTAAAACTCTGTAAACATC-3′ |
a
The oligonucleotides used for site-directed mutagenesis represent the complement of the coding strand of the VP7 gene. The underlined triplets are the altered anticodons.