TABLE 2.

VP7 mutants and their allocated chemical and functional features

VP7 mutant	Location of lower domain	Substitution	Charge	Side chain	Chemical descriptiona	Hydropathicityb
E104W	Helix 5	Glu104 to Trp	Acidic to nonpolar	Aromatic	−→0	Phil to Phob
R111F	Helix 5	Arg111 to Phe	Basic to nonpolar	Aromatic	+→0	Phil to Phob
W119D	Loop between helices 5 and 6	Trp119 to Asp	Nonpolar	Aromatic to acidic	0→−	Phob to Phil
F268D	Helix 6	Phe268 to Arg	Nonpolar	Aromatic to basic	0→+	Phob to Phil
Y271R	Helix 6	Tyr271 to Arg	Unchc-polar	Aromatic to basic	−→0	Phob to Phil
D318N	Helix 8	Asp318 to Asn	Acidic to unch-polar	Amide	−→0	Phil to Phob
T321R	Helix 8	Thr321 to Arg	Unch-polar	Hydroxyl to basic	0→+	Phob to Phil

^aData show that mutations resulted in changes from a negatively (−) or positively (+) charged or neutral (0) state. 

^bPhil, hydrophilic; Phob, hydrophobic. 

^cUnch, uncharged.