Table 3.
Curing in LAB strains harboring more than two plasmids.
| Scientific name | Strain | Agent / (μg/ml) | Incubation time (h) | Plasmid 1 cured colonies | Plasmid 2 cured colonies | Plasmid 3 cured colonies | Plasmid 1&2 cured colonies | Plasmid 1&3 cured colonies | Plasmid 2&3 cured colonies | Plasmid 1&2&3 cured colonies |
|---|---|---|---|---|---|---|---|---|---|---|
| Lactiplantibacillus plantarum | DS1989 | Nv / 10 | 72 | 15/96 (16%) | 9/96 (9%) | 1/96 (1%) | 1/96 (1%) | 0/96 | 0/96 | 0/96 |
| Nv / 50 | 72 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| EtBr / 50 | 72 | 1/96 (1%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| EtBr/ 10 | 72 | 1/96 (1%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| AO / 10 | 72 | 2/96 (2%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| AO / 50 | 72 | 2/96 (2%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| Lactiplantibacillus plantarum | DS0815 | Nv / 10 | 72 | 91/96 (95%) | 4/96 (4%) | 4/96 (4%) | 4/96 (4%) | 4/96 (4%) | 0/96 | 0/96 |
| Nv / 50 | 72 | 6/6 (100%) | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | ||
| EtBr / 10 | 72 | 9/96 (9%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| EtBr/ 50 | 72 | 18/22 (82%) | 1/22 (5%) | 0/22 | 0/22 | 0/22 | 0/22 | 0/22 | ||
| AO / 10 | 72 | 9/96 (9%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| AO / 50 | 72 | 7/96 (7%) | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | 0/96 | ||
| Lactiplantibacillus plantarum | DS1073 | Nv / 10 | 72 | 54/96 (56%) | 0/96 | - | 0/96 | - | - | - |
| Nv / 50 | 72 | 9/96 (9%) | 0/96 | - | 0/96 | - | - | - | ||
| EtBr / 10 | 72 | 1/96 (1%) | 0/96 | - | 0/96 | - | - | - | ||
| EtBr / 50 | 72 | 19/96 (20%) | 0/96 | - | 0/96 | - | - | - | ||
| AO / 10 | 72 | 1/96 (1%) | 0/96 | - | 0/96 | - | - | - | ||
| AO / 50 | 72 | 0/96 | 0/96 | - | 0/96 | - | - | - | ||
| Lactiplantibacillus plantarum | DS1902 | Nv / 10 | 72 | 0/96 | 16/96 (17%) | - | 0/96 | - | - | - |
| Nv / 50 | 72 | 0/96 | 1/96 (1%) | - | 0/96 | - | - | - | ||
| EtBr / 10 | 72 | 0/96 | 0/96 | - | 0/96 | - | - | - | ||
| EtBr / 50 | 72 | 0/96 | 0/96 | - | 0/96 | - | - | - | ||
| AO / 10 | 72 | 0/96 | 0/96 | - | 0/96 | - | - | - | ||
| AO / 50 | 72 | 0/96 | 0/96 | - | 0/96 | - | - | - |
–, the strain does not possess plasmid 3. EtBr, Ethidium bromide; AO, Acridine orange; Nv, Novobiocin.