Table 4.

Plasmid curing efficiency based on plasmid size.

Strain	Number of plasmids	Plasmid size (bp)	Copy number	G+C content (%)	Agent / (μg/ml)	Incubation time (h)	Cured colonies	Curing (%)
DS1989	3	50,933	38.9	38.9	EtBr/ 10	72	1/96	1
					EtBr / 50	72	1/96	1
					AO / 10	72	2/96	2
					AO / 50	72	2/96	2
					Nv / 10	72	15/96	15
		48,573	121.5	39.0	Nv / 10	72	9/96	9
		8,694	142.2	36.0	Nv / 10	72	1/96	1
DS0815	3	66,660	4.3	39.3	EtBr/ 10	72	9/96	9
					EtBr / 50	72	18/22	82
					AO / 10	72	9/96	9
					AO / 50	72	7/96	7
					Nv / 10	72	91/96	95
					Nv / 50	72	6/6	100
		39,431	3.6	40.5	EtBr / 50	72	1/22	5
					Nv / 10	72	4/96	4
		6,156	8.6	37.4	Nv / 10	72	4/96	4
DS1073	2	50,512	1.9	39.0	EtBr / 10	72	1/96	1
					EtBr / 50	72	19/96	20
					AO / 10	72	1/96	1
					Nv / 10	72	54/96	56
					Nv / 50	72	9/96	9
		19,584	4.2	40.5	ALL	72	0/96	0
DS1902	2	7,845	3.4	36.9	ALL	72	0/96	0
		2,410	9.8	38.2	Nv / 10	72	16/96	17
					Nv / 50	72	1/96	1
DS0725	1	66,795		43.8	EtBr / 50	72	5/96	5
DS2766	1	6,196		38.9	EtBr / 10	24	4/96	4
					EtBr / 50	24	4/96	4
					EtBr / 10	48	7/96	7
					EtBr / 50	48	21/96	22
					AO / 10	48	17/96	18
					AO / 50	48	21/96	22

EtBr, Ethidium bromide; AO, Acridine orange; Nv, Novobiocin.