FIG. 4.

Effects of inhibitors of PLA₂ and cyclooxygenase on DEN-2 virus-induced apoptosis. SK-N-SH cells were pretreated with various inhibitors as indicated for 2 h and were infected with DEN-2 PL046 virus at an MOI of 5. Apoptotic cells were determined at 60 h p.i., and the inhibitors were maintained in the culture medium continuously for the duration of the experiment. (A) PLA₂ inhibitors, AACOCF₃ (10 μM) and ONO-RS-082 (10 μM), protected cells against DEN-2 virus-induced apoptosis. (B) Cyclooxygenase inhibitors, aspirin (5 mM) and indomethacin (10 μM), enhanced DEN-2 virus-induced apoptosis. The values represent the means ± SD of triplicate measurements from one of three similar experiments, and the asterisks indicate a significant difference between the absence and presence of inhibitor (P < 0.01 by Student's t test).