FIG. 5.

Release of cytochrome c from mitochondria in response to DEN-2 virus infection and exogenous AA. SK-N-SH cells were infected with DEN-2 PL046 virus at an MOI of 5 (A) or treated with exogenous AA at a concentration of 100 μM (B). At the indicated times, cytosols were isolated and the presence of cytochrome c was detected by Western immunoblotting analysis using monoclonal antibody specific to cytochrome c. The levels of actin in the cytosols were demonstrated as an internal control. (C) Mitochondrial fractions were isolated from uninfected SK-N-SH cells and treated with AA (100 μM) in the presence or absence of cytosol for 30 min. Cytochrome c released into the buffer was detected as described above.