Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1976 Jun 15;156(3):569–575. doi: 10.1042/bj1560569

Evidence for the lack of deoxyribonucleic acid dark-repair in Halobacterium cutirubrum.

V L Grey, P S Fitt
PMCID: PMC1163790  PMID: 949339

Abstract

1. Halobacterium cutirubrum does not perform dark-repair of DNA either after u.v. irradiation or during normal growth. 2. Cultures irradiated with u.v. are readily photoreactivated, but do not recover viability in the dark. 3. No increase in the rate of DNA synthesis is observed in the surviving cells after u.v. irradiation. 4. At early times during normal semiconservative replication, newly incorporated thymidine is found only in the hybrid DNA. 5. It is suggested that these bacteria may be useful in the study of DNA replication and photoreactivation.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clayton D. A., Doda J. N., Friedberg E. C. The absence of a pyrimidine dimer repair mechanism in mammalian mitochondria. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2777–2781. doi: 10.1073/pnas.71.7.2777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Furano A. V. A very rapid method for washing large numbers of precipitates of proteins and nucleic acids. Anal Biochem. 1971 Oct;43(2):639–640. doi: 10.1016/0003-2697(71)90300-9. [DOI] [PubMed] [Google Scholar]
  3. Gochnauer M. B., Kushner D. J. Growth and nutrition of extremely halophilic bacteria. Can J Microbiol. 1969 Oct;15(10):1157–1165. doi: 10.1139/m69-211. [DOI] [PubMed] [Google Scholar]
  4. Grey V. L., Fitt P. S. An improved synthetic growth medium for Halobacterium cutirubrum. Can J Microbiol. 1976 Mar;22(3):440–442. doi: 10.1139/m76-068. [DOI] [PubMed] [Google Scholar]
  5. HUTCHISON W. C., MUNRO H. N. The determination of nucleic acids in biological materials. A review. Analyst. 1961 Dec;86:768–813. doi: 10.1039/an9618600768. [DOI] [PubMed] [Google Scholar]
  6. Harris W. J. The occurrence of two types of synthesis of deoxyribonucleic acid during normal growth in Bacillus subtilis. Biochem J. 1973 Oct;135(2):315–325. doi: 10.1042/bj1350315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hescox M. A., Carlberg D. M. Photoreactivation in Halobacterium cutirubrum. Can J Microbiol. 1972 Jul;18(7):981–985. doi: 10.1139/m72-152. [DOI] [PubMed] [Google Scholar]
  8. Howard-Flanders P. DNA repair and recombination. Br Med Bull. 1973 Sep;29(3):226–235. doi: 10.1093/oxfordjournals.bmb.a071012. [DOI] [PubMed] [Google Scholar]
  9. Huang A. T., Kremer W. B., Laszlo J., Setlow R. B. DNA repair in human leukaemic lymphocytes. Nat New Biol. 1972 Nov 22;240(99):114–116. doi: 10.1038/newbio240114a0. [DOI] [PubMed] [Google Scholar]
  10. Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moore R. L., McCarthy B. J. Base sequence homology and renaturation studies of the deoxyribonucleic acid of extremely halophilic bacteria. J Bacteriol. 1969 Jul;99(1):255–262. doi: 10.1128/jb.99.1.255-262.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterkin P. I., Fitt P. S. Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum polynucleotide phosphorylase. Biochem J. 1971 Feb;121(4):613–620. doi: 10.1042/bj1210613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Setlow R. B., Setlow J. K. Effects of radiation on polynucleotides. Annu Rev Biophys Bioeng. 1972;1:293–346. doi: 10.1146/annurev.bb.01.060172.001453. [DOI] [PubMed] [Google Scholar]
  14. Setlow R. B. The photochemistry, photobiology, and repair of polynucleotides. Prog Nucleic Acid Res Mol Biol. 1968;8:257–295. doi: 10.1016/s0079-6603(08)60548-6. [DOI] [PubMed] [Google Scholar]
  15. Sinclair J. H. Use of preparative CsCl gradients to estimate DNA buoyant densities: potential errors due to fractionation artifacts. Anal Biochem. 1974 Jan;57(1):240–254. doi: 10.1016/0003-2697(74)90070-0. [DOI] [PubMed] [Google Scholar]
  16. Smith K. C., O'Leary M. E. The pitfalls of measuring DNA synthesis kinetics as exemplifed in ultraciolet radiation studies. Biochim Biophys Acta. 1968 Dec 17;169(2):430–438. doi: 10.1016/0005-2787(68)90051-8. [DOI] [PubMed] [Google Scholar]
  17. Werner R. Nature of DNA precursors. Nat New Biol. 1971 Sep 22;233(38):99–103. doi: 10.1038/newbio233099a0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES