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Experimental design
| Rabbit group (no. of rabbits) | DNA treatmenta
|
|||
|---|---|---|---|---|
| Priming agent
|
Vaccine agent
|
|||
| GM-CSF | Plasmid | E6 | Plasmid | |
| Control (3) | − | pcDNA3 | − | pCMV-β |
| E6 only (4) | − | pcDNA3 | + | pdCMV-E6 |
| GM-CSF only (6) | + | pPJV3226 | − | pCMV-β or pcDNA3 |
| GM-CSF + E6 (6) | + | pPJV3226 | + | pdCMV-E6 |
Vector pcDNA3 contains no insert; pPJV3226 encodes mouse GM-CSF; pCMV-β encodes β-galactosidase; pdCMV-E6 encodes the full-length CRPV E6 protein (55).
