Table 2.
Primer sequences utilized for gene expression analysis via RT-qPCR assays.
| Specificity/Target gene | Primer sequence (5′-3′) | Accession No./Reference |
|---|---|---|
| Inflammatory mediators | ||
| IL-1β | F: TGACAGACCCCAAAAGATTAAGG R: CTCATCTGGACAGCCCAAGTC |
NM_031512.2 |
| IL-6 | F: CCACCAGGAACGAAAGTCAAC R: TTGCGGAGAGAAACTTCATAGCT |
NM_012589.2 |
| IL-18 | F: ATGGCTGCCATGTCAGAAGA R: TTGTTAAGCTTATAAATCATGCGGCCTCAGG |
XM_039080945.1 |
| IL-10 | F: GCCCAGAAATCAAGGAGCATT R: CAGCTGTATCCAGAGGGTCTTCA |
L02926.1 |
| TNFα | F: CAGCCGATTTGCCATTTCA R: AGGGCTCTTGATGGCAGAGA |
L19123.1 |
| Antioxidant markers | ||
| CAT | F: ACGAGATGGCACACTTTGACAG R: TGGGTTTCTCTTCTGGCTATGG |
NM_012520.2 |
| SOD | F: AGCTGCACCACAGCAAGCAC R: TCCACCACCCTTAGGGCTCA |
NM_017051.2 |
| GSH-Px | F: AAGGTGCTGCTCATTGAGAATG R: CGTCTGGACCTACCAGGAACT |
NM_030826.4 |
| HO-1 | F: CCCAGAGGCTGTGAACTCTG R - AGGCCCAAGAAAAGAGAGCC |
NM_012580.2 |
| Nrf2 | F: GGTTGCCCACATTCCCAAAC R - GGCTGGGAATATCCAGGGCA |
NM_031789.2 |
| NQO1 | F: CATTCTGAAAGGCTGGTTTGA R: CTAGCTTTGATCTGGTTGTCAG |
(Yeligar et al., 2010) |
| Angiogenic and wound healing markers | ||
| TGF-β1 | F: CCAGCCGCGGGACTCT R: TTCCGTTTCACCAGCTCCAT |
NM_021578.2 |
| VEGF | F: GATCCAGTACCCGAGCAGTCA R: TCTCCTTTCTTTTTGGTCTGCAT |
NM_053549.1 |
| Ang-1 | F: AGGTTGGTGGTTTGATGCCT R: CGGGAACATCCCCAGATTGT |
NM_017232 |
| MMP-9 | F: GACACCACCGAGCTATCCAC R: TTTAAACGGGCTGTTTCCCCT |
(Zhao et al., 2023) |
| Collagen I | F: TTTGGAGAGAGCATGACCGA R: AGGGACTTCTTGAGGTTGCC |
(Zhao et al., 2023) |
| Collagen III | F: TGCAATGTGGGACCTGGTTT R: GGGCAGTCTAGTGGCTCATC |
(Zhao et al., 2023) |
| Ki-67 | F: GGGTTTCCAGACACCAGACC R: CCAGGAAGACCAGTTAGAACC |
NM_001271366.1 |
| EGF | F: CTCAGGCCTCTGACTCCGAA R: ATGCCGACGAGTCTGAGTTG |
NM_012842.1 |
| bFGF | F: CGATAGAACACGGCATCAaTC R: CATCAGGCAGTTCGTAGCTC |
NM_019305.2 |
| C. albicans biofilm | ||
| ALS1 | F: CCATCACTGAAGATATCACCACA R: TGGAGCTTCTGTAGGACTGGTT |
(Cheng et al., 2005) |
| HYR1 | F: TTGTTTGCGTCATCAAGACTTTG R: GTCTTCATCAGCAGTAACACAACCA |
(Tsang et al., 2012) |
| PLB1 | F: GGTGGAGAAGATGGCCAAAA R: AGCACTTACGTTACGATGCAACA |
(Nailis et al., 2010) |
| Housekeeping genes | ||
| β-actin | F: CGCAGTTGGTTGGAGCAAA R: ACAATCAAAGTCCTCAGCCACAT |
V01217.1 |
| EFB1 | F: TCAGATTTCTCTAAAGTCG R: TGACATCAGCTTGAGTGG |
X96517 |
IL, interleukin; TNF-α, tumor necrosis factor-alpha; CAT, catalase; SOD, superoxide dismutase; GSH-Px, glutathione peroxidase; HO-1, heme oxygenase-1; Nrf2, nuclear factor erythroid 2-related factor 2; NQO1, NAD(P)H quinone oxidoreductase 1; TGF-β1, transforming growth factor beta 1; VEGF, vascular endothelial growth factor; Ang-1, angiopoietin-1; MMP-9, matrix metalloproteinase-9; Ki-67, Kiel-67; EGF, epidermal growth factor; bFGF, basic fibroblast growth factor; ALS1, C. albicans agglutinin-like sequence 1; HYR1, hyphally regulated gene; PLB1, phospholipase B; EFB1, elongation factor 1-beta.