Northern blot analysis of PCV RNAs with GAC, GΔC, and CCA anticodons amplified in N. benthamiana plants. The indicated RNA1 + RNA2 combinations (as in Fig. 3) were inoculated onto N. benthamiana plants. Total RNAs were extracted at 11 dpi and separated on a 1% agarose gel, and the positive strands of the viral RNA genome were detected by Northern hybridization as in Fig. 3. The positions of RNAs 1 and 2 are indicated at left. The relative accumulations of RNA1 and RNA2, as well as the RNA1/RNA2 ratios, are shown beneath each lane.