TABLE 1.

Direct competition between RNA1 and RNA2 variants with different anticodon sequences in N. benthamiana plants^a

RNA in competitionb	Anticodon sequence		RNA ratio (WT/mutant)
	WT	Mutant	Inoculated leaves	Apical leaves
RNA1	1GAC	1GΔC	1.4	2.4
	1GAC	1CCA	8.1	Only GACc
RNA2	2GΔC	2GAC	0.14	Only GACc
	2GΔC	2CCA	0.70	0.45

^aRNAs were extracted 11 dpi, polyadenylated, and subjected to RT-PCR, amplifying the 3′ 234 nt, which were sequenced by the fluorescent dye terminator method (see Fig. 5). The ratios of anticodon sequences were determined as described in the text. WT, wild type. 

^bThe indicated variants of RNA1 were coinoculated with wild-type RNA2 (GΔC). The indicated variants of RNA2 were coinoculated with wild-type RNA1 (GAC). 

^cThe detection limit for a minor sequence is about 10% of the predominant sequence.