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. 2024 Nov 11;265(1):14–25. doi: 10.1002/path.6363

Figure 1.

Figure 1

AMIGO2 is upregulated in CAFs compared to NAFs. (A) AMIGO2 reads from bulk RNA‐seq of normal tissue‐associated fibroblasts (NAFs) and CAFs isolated from CRC patients. Results are given as mean ± SD of read values. A two‐tailed unpaired Student's t‐test was used for statistical evaluation (*p = 0.0175). (B) Comparison of AMIGO2 mRNA expression in paired NAFs and CAFs from the same patients (n = 8) each. RPL37A was used as a reference transcript for normalization. Results are given as 40 – ΔCt (Ct‐AMIGO2 – Ct‐RPL37A) (mean ± SD, t‐test, **p = 0.0025). (C) AMIGO2 western blot of paired NAFs and CAFs from the same patients as in (B). GAPDH was used as a loading control. (D) Quantitative evaluation of western blot results from (C). Results are shown as mean ± SD (t‐test, **p = 0.0267, left) or by pairwise comparison for each patient (right). (E) Staining for AMIGO2 and the fibroblast marker vimentin in consecutive tissue sections of human CRC tissues. Double‐positive fibroblasts are indicated by white arrows. (F) AMIGO2 immunohistochemistry on normal and CRC tissues (n = 10). Patient 1 images are also used in supplementary material, Figures S2. (G) Quantification of stromal staining intensity between normal and CRC tissues (mean ± SD, two‐tailed, unpaired Student's t‐test, *p < 0.05).