FIG. 6.

Peptide epitope-specific CD4⁺ T cells detected by tetramer binding in peptide-stimulated PBMCs of SHIV-infected rhesus monkeys and recombinant canarypox virus-vaccinated monkeys. p46-specific CD4⁺ T cells among in vitro p46-stimulated PBMCs from SHIV-infected Mamu-DR*W201⁺ monkeys (A) and recombinant canarypox virus-vaccinated Mamu-DR*W201⁺ rhesus monkeys (B) were identified by their binding to tetrameric Mamu-DR*W201–p46 complex. p46-stimulated PBMCs from 10 infected and 10 vaccinated rhesus monkeys were stained with PE-coupled tetrameric Mamu-DR*W201–p46 complex. Flow-cytometric analysis was performed on gated CD4⁺ CD3⁺ T cells.