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. 2024 Oct 28;32(12):4448–4466. doi: 10.1016/j.ymthe.2024.10.016

Figure 3.

Figure 3

Antigen recall responses assessed in splenocytes and cervical lymph node isolates from IM/IN immunized mice demonstrate enhanced Th1/Th17 profiles

Splenocytes (A–F) and cLN cellular isolates (G–L) were prepared from mice given prime/boost immunizations with the indicated adjuvant/antigen regimens two weeks after the final immunization (week 6). Cells were stimulated ex vivo with 5 μg S protein for 72 h, and levels of secreted (A, G) IFN-γ, (B, H) IL-2, (C, I) TNF-α, (D, J) IL-6, (Ε, Κ) IL-17A, and (F, L) IL-10 were measured from splenocytes and cLN respectively, by multiplex immunoassay relative to unstimulated cells. (n = 4–5/group; ∗p < 0.05, ∗∗p < 0.01 by Mann-Whitney U test shown only for select groups-(full statistical analysis is shown in Table S1).