Figure 4.

Induced CD1c⁺ B cells inhibited the proliferation of PBMCs and pro-inflammatory cytokine secretion of T cells

IL-10-producing B cells were induced by db-cAMP and CHIR-99021 in the presence of CpG. Then, CD19⁺CD1c⁺ B cells and CD19⁺CD1c⁻ B cells were sorted and cocultured with PBMCs or CD3⁺ T cells. (A) Representative histograms of CellTrace Violet-labeled PBMC proliferation with or without B cells in the presence or absence of anti-IL-10 neutralizing antibody (IL-10 NAb), and the gray histogram is the unstimulated PBMC as control. (B) Representative plots of TNF-α production by T cells in the presence or absence of IL-10 NAb. (C) Representative plots of IFN-γ production by T cells in the presence or absence of IL-10 NAb. (D–F) The quantification of CellTrace Violet dilution (D), TNF-α-producing T cells (E), and IFN-γ-producing T cells (F). Data represent mean ± SEM of three independent experiments; not significant (ns) p ≥ 0.05; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.