Fig. 3.

DF-prepared MMIX compared with manually-prepared MMIX after addition of 0.05% Tween-20. endpoint RFU data from PCR reactions using dilutions of synthetic RNA positive control material (Twist) at and around the limit of detection. (A) ROX RFU kernel distribution and RFU counts. Dotted line represents the 1600 ROX RFU cutoff. Dashed lines represent the arithmetic mean of ROX RFU. (B) Kernel Distribution and count for FAM RFU values. Dashed lines represent the arithmetic mean of FAM RFU. (C) Kernel Distribution and count for n_FAM values. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dashed lines represent the arithmetic mean of n_FAM. (D) Scatterplot n_FAM vs. ROX RFU. Solid lines represent the “Inconclusive” PLOD (Blue) and “Detected” (Black) n_FAM cutoff for clinical calling. Dotted line represents the 1600 ROX RFU cutoff. Data are pooled from seven 384-well arrays for both conditions. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)