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TABLE 1.

PCR primers for detection and sequencing of insertions in NS2-3

Primer set Forward primer
Reverse primer
Sequence Positiona Sequence Positiona
A ATG-ACC-ACC-ATG-TGG-GC 4244 GAG-TTG-TCC-TCT-GGC-TAT-GT 5029
B GTG-TGG-AAG-ACA-AGG-AAA-GC 4894 TTG-GTA-GGT-TCC-TCA-GGA-AG 5069
C AGT-GAG-AAA-TGA-AGC-CGT-CC 4730 TGT-ACC-TCT-AGG-CAT-GAT-CC 5234
a

Nucleotide position numbers correspond to the sequence of BVDV1-SD-1 (GenBank accession number M96751).