Figure 3.

Molecules with antimicrobial function are impacted in IL-17RA deficient mice during H. pylori infection. A. Realtime rtPCR was performed on gastric tissue at 6 weeks post-infection for S100a8, a component of calprotectin. Expression is presented relative to the housekeeping gene Gapdh and to uninfected InsGAS mice (calibrator sample). B. PMN infiltration was scored as acute inflammation on a scale of 0-6 at this same time point. C. Realtime rtPCR was performed on gastric tissue at 6 weeks post-infection for Muc5ac and Muc6. Expression is presented relative to the housekeeping gene Gapdh and to uninfected InsGAS mice (calibrator sample). D. Immunohistochemical stainings for Muc5ac were performed on tissues at 3 mo. post-infection. Representative images (200×) are from 2 different mice per genotype. Error bars represent ± SEM; *p ≤ 0.05, **p ≤ 0.01.