FIG. 3.

Test for preservation of retroviral sequences in transduced cells. Total cellular DNAs were prepared from G418-resistant PG13 cells transduced with retroviral vectors as described by Kim et al. (11). PCR was performed with 5 μg of total genomic DNA and oligonucleotide primers specific to various regions of the retroviral vector as indicated. The two pairs of oligonucleotide primers used to amplify the retroviral regions were described in the Fig. 1 legend.