TABLE 2.
Vector | Relative CAT activity
|
Viral titer (105) | ||
---|---|---|---|---|
Producer line | Transduction
|
|||
Transient | Stable | |||
MSN | 100 | 100 | 100 | 3.2 ± 1.5 |
MSNΔ17 | 83 ± 6 | 7.1 ± 2.7 | 85 ± 10 | 0.14 ± 0.06 |
Cell-free viral supernatants were prepared as described in Table 1 and were used to transduce the PG13 line (13) at the same low MOI, around 0.1. All other procedures were identical to those described in Table 1 except that HT1080 cells, instead of NIH 3T3 cells, were used as target cells, as they are prone to viruses pseudotyped with GaLV env.