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. 1976 Jul 15;158(1):23–31. doi: 10.1042/bj1580023

Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro.

N De Groot, I J Yuli, H Czosnek, Y Shiklosh, A A Hochberg
PMCID: PMC1163932  PMID: 9077

Abstract

The amino acid-incorporating activities of free polyribosomes, rough membranes and rough membranes reconstituted in vitro, derived from rat liver, were compared. The amino acid-incorporating activity of the two membrane fractions were very similar in their response towards changes in pH, Mg2+ concentration and temperature, but differed from the response of the amino acid-incorporating activity of free polyribosomes. Free polyribosomes irreversibly lost part of their amino acid-incorporating capacity after they had become bound to rough membrane, from which the original ribosomes had been removed. Ribonuclease activity present in the membrane fraction may be responsible for this loss.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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